Table 4.
Effect of flavonolignans and silymarin on elastase and collagenase activity.
| Compounds | Elastase | Collagenase | ||
|---|---|---|---|---|
| IC50 (µM) | IC50 (mg/L) | IC50 (µM) | IC50 (mg/L) | |
| SB | 122.6 ± 4.8 # | 59.1 ± 2.3 * | 52.2 ± 5.0 # | 25.2 ± 2.4 * |
| DHSB | 8.6 ± 0.5 # | 4.1 ± 0.2 * | 23.4 ± 2.9 # | 11.2 ± 1.4 * |
| ISB | ~ | ~ | 50.8 ± 4.5 # | 24.5 ± 2.2 * |
| SD | ~ | ~ | 190.3 ± 4.4 # | 91.8 ± 2.1 * |
| SC | ~ | ~ | 95.9 ± 5.7 # | 46.3 ± 2.8 * |
| SM | - | 6.27 ± 0.4 | - | 2.0 ± 0.3 |
| OA | 10.8 ± 0.6 | 4.9 ± 0.3 * | N.D. | N.D. |
| 1,10-Ph | N.D. | N.D. | 161.3 ± 4.6 | 29.1 ± 0.8 * |
Silybin (SB), dehydrosilybin (DHSB), isosilybin (ISB), silydianin (SD), silychristin (SC), silymarin (SM). Oleanolic acid (OA) is a standard inhibitor of elastase. 1,10-Phenantroline (1,10-Ph) is a specific inhibitor of collagenase. ~ IC50 could not be determined due to the limited solubility of the studied compounds. N.D. IC50 was not determined for this compound. - SM is a mixture of substances, and the molar concentration of the extract is impossible to evaluate. # Significantly different from the respective standard inhibitor at p = 0.05; * significantly different from SM at p = 0.05.