Table 2.
Kinetic parameters for Thg1 on variant tRNAHis substrates.
| tRNAHis+CCA | tRNAHis∆CCA | tRNAHis+CCA A73C | tRNAHis∆CCA A73C | |
|---|---|---|---|---|
|
kobs
(min−1) |
0.014 ± 0.001 | 0.0070 ± 0.0004 | 0.097 ± 0.008 | 0.013 ± 0.001 |
|
KD,app,tRNA
(µM) |
5.8 ± 4.3 | >30 * | 3.5 ± 1.7 | >30 * |
|
kmax/KD,app
(µM−1min−1) |
385 ± 45 | ND | 110 ± 7.7 | ND |
Data are means ± SD; n = 3; ND: not determined. * saturation was not reached even at the highest possible concentration of purified protein in the assays, leading to the lower estimate for KD,app of 30 µM