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The mutant binding parameters: The affinities and stoichiometries were determined by fitting the saturation curves of bound radioactive [3H]-PK 11195 to rec-mTSPO reconstituted in proteoliposomes.
| Mutation | Kd (nM) | Bmax (nmol/mg) |
|---|---|---|
| WT | 8 ± 3 | 25 ± 3 |
| Y34S | 40 ± 7 | 25 ± 5 |
| Y34F | 20 ± 3 | 27 ± 3 |
| Δ41–50 | 20 ± 8 | 25 ± 8 |
| ΔC-ter (153–169) | 20 ± 5 | 30 ± 10 |
| F100A | 20 ± 5 | 25 ± 7 |
| Y34F/F100A | 150 ± 20 | 25 ± 10 |
| Y34F/F99A | 150 ± 20 | 25 ± 10 |
