Figure 5.

Effect of FAK inhibitor, PF-562271, on (+)-rhodoptilometrin-promoted wound healing in the hGF-1 cell. (A) After prior incubation with PF-562271 (10 μM) for 2 h, the cells were treated with scratch and (+)-rhodoptilometrin (10 μM) for 0, 12, and 24 h, and then photographed by phase-contrast microscopy at 100× magnification. (B) Scratch-test assay statistics of the remaining wound area were normalized with the time point 0 h. Data (means ± SEM) are representative of at least three independent experiments. Student’s t-test determined the significance; * p < 0.05, ** p < 0.01, compared with untreated cells in same time point.