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. 2001 Nov 20;98(25):14487–14492. doi: 10.1073/pnas.251304398

Figure 2.

Figure 2

Pattern of wg and DWnt2 expression with respect to Sal protein and the tracheal cells. (A and B) Three tracheal metameres of a 1-eve-1 embryo at stage 10 (A) or 12 (B) stained with a riboprobe for DWnt2 (green) and for Sal (in red) and β-Gal (blue) for the tracheal cells. Note that DWnt2 is expressed near the tracheal cells during stage 10 (A) in cells that also express sal. (C and D) Three tracheal metameres of a 1-eve-1 embryo at stage 10 (C) or 12 (D) stained with a riboprobe for wg (green) and for Sal (in red) and β-Gal (blue) for the tracheal cells. (E) Two tracheal metameres of a 1-eve-1 embryo at stage 11 stained with a riboprobe for DWnt2 (green) and for Wg (red) and β-Gal (blue) for the tracheal cells. Note that wg and DWnt2 differ in their pattern of expression. (F) Diagram summarizing the stainings in A–E. DWnt2 is expressed in the dorsal ectoderm near the tracheal cells that is in the most dorsal Sal-positive cells. wg expression alternates in stripes with Sal protein. Both genes are produced near the tracheal cells.