Figure 3.
Tracheal requirements of wg and/or DWnt2. (A–C) Lateral views of embryos at late stages of embryogenesis stained with mAb2A12 to highlight the lumen of the tracheae. (A and B) wg−DWnt2− double mutants, the DT is missing (A) or very much reduced (arrow in B). The arrowhead in A points to an incomplete VB. (C) DWnt2 combination, no tracheal defects are observed. Arrow points to DT. (D–I) Sal distribution at stages 12 (D–F) and 14/15 (G–I) in lateral views of embryos of the indicated genotypes. Note the absence of Sal in wg−DWnt2− mutants (E, H, and Insets) as compared with WT (D, G, and Insets). Low levels of Sal still are observed in wg− mutants (F, I, and Insets). (J and K) dpp expression at stage 11 in lateral views of embryos of the indicated genotypes. (L and M) Kni distribution at stage 14/15 in lateral views of embryos of the indicated genotypes.
