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Binding parameters of 2-FAL on MIP determined by the D-shaped POF-MIP-SPR sensor SPR-MIP by Scatchard plot, and sensing parameters.
| KA (M−1) | kcR (nm) | Sensitivity at Low Concentration (nm/M) | LOD (M)1 | |
|---|---|---|---|---|
| Sites at high affinity (sites 3) (c up to 6 10−7 M) |
3.0(4) 107 | 7(2) | 2.0(2) 108 | 5 × 10−9 |
| Sites at low affinity (sites 2) (c =3-60 10−6 M) |
1.7(2) 105 | 6(1) | 9.7(6) 105 |
1 LOD obtained by dividing the resolution of the spectrometer (1.5 nm) by the sensitivity at low concentration.
