Skip to main content
. 2018 Dec 10;20(4):595–604. doi: 10.1002/cbic.201800526

Figure 2.

Figure 2

The 22 nt long F‐tag RNA sequence and complementary FIT probes, and the corresponding absorption and emission spectra of probes in the absence (‐ ‐ ‐ ‐) and presence (—) of F‐tag RNA. Small letters indicate PNA monomers; capital letters represent nucleotides. Underlined nucleotides mark 2′‐OMe‐modified nucleotides. Subscript L highlights a locked nucleic acid (LNA) building block. X: (CH2)2CO−NH(CH2)5−CO. Conditions: 0.5 μm probe with target RNA (5 equiv) in phosphate‐buffered saline (PBS; 100 mm NaCl, 10 mm Na2HPO4, pH 7) at 37 °C. λ ex=485 nm, λ em=500–650 nm, slitex=5 nm, slitem=5 nm.