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Schematic description of the cloning procedure used for the construction of a repeat motif (=F‐tag) appended to the 3′‐UTR of mCherry mRNA. BglII and BamHI: cut sites for restriction enzymes; attR1, attR2, attL1, attL2, attL3, attL4, attB1, attB2, attB3, and attB4: recombination sites; km: kanamycin resistance gene; amp: ampicillin resistance gene; CMV‐TO: doxycycline inducible CMV promoter (Tet‐ON); mCherry: fluorescent protein; FLP: recombinase flippase; FRT: FLP recognition target.
