Table 1.

Optical properties of FIT probes and FIT probe conjugates.

	Probe	F 0 [a]	F [b]	F/F 0	ϵ max [c]	T m [d]
1	FIT‐DNA1	4.9	142	29	85 640	49
2	Pen_FIT‐DNA1	4.7	140	30	75 780	49
3	SAP(E)_FIT‐DNA1	3.8	110	29	64 780	44
4	R8+FIT‐DNA1	5.7	161	28	85 640	60
5	Palm_FIT‐DNA1	9.7	115	12	57 170	49
6	FIT‐PNA1	2.7	22	8	41 790	52
7	Pen_FIT‐PNA1	15	68	4.5	64 310	58
8	SAP(E)_FIT‐PNA1	9.9	45	4.5	45 460	56
9	Pep2_FIT‐PNA1	11	68	6	62 320	53
10	Transp_FIT‐PNA1	3.2	11	3.5	43 460	49
11	MPG_FIT‐PNA1	2.5	12	5	40 620	52

[a] Fluorescence intensity of a single strand. [b] Fluorescence intensity after hybridization with F‐tag‐RNA (Figure 2). [c] Extinction coefficient at the TO absorption maximum. [d] Melting temperature of a double strand at 0.5 μm. Conditions: 0.5 μm probes, complementary RNA (5 equiv, if added) in PBS (100 mm NaCl, 10 mm Na₂HPO₄, pH 7) at 37 °C, λ _ex=488 nm, λ _em=535 nm.