Table 2.

Primers used for the validation of microarray results.

Gene Symbol	Primers Sequences	Reaction Conditions		Primer (nM)	Target Sequence Accession Number	References
CGA	F: 5′-CTCCAGAGCGTACCCAACTC-3′ R: 5′-ACTGTGGCCTTGGTAAATGC-3′	Activation: 50 °C, 30 min;	40 cycles	500 nM	XM_005659277.1	[60]
		95 °C 15 min,
		1. Denaturation: 94 °C, 15 s
		2. Annealing: 55 °C, 30 s
		3. Extension: 72 °C, 30 s
		77 °C, 15 s
CXCL8	F: 5′-GGCAGTTTTCCTGCTTTCT-3′ R: 5′-CAGTGGGGTCCACTCTCAAT-3′	Activation: 95 °C, 15 min	40 cycles	400 nM	X61151.1	[61]
		1. Denaturation: 94 °C, 15 s
		2. Annealing: 58 °C, 30 s
		3. Extension: 72 °C, 30 s
FGF7/KGF	F: 5′-GCTTCCACATTATCTGTCTGGTG-3′ R: 5′-GTCCCTTTGACTTTGCCTCG-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	AF217463.1	This study
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
HSD3B1	F: 5′-AGGTTCGCCCGCTCATC-3′ R: 5′-CTGGGCACCGAGAAATACTTG-3′	Activation: 95 °C, 10 min	40 cycles	300 nM	NM_001004049.1	[62]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 61 °C, 1 min
		3. Extension: 72 °C, 1 min
HSD17B8	F: 5′-TTCTGCTCCGCATGTCTGAAG-3′ R: 5′-CCATGTTTCCCACCTTCCCTA-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	NM_001130730.1	This study
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
IL1B	F: 5′-TGCCAACGTGCAGTCTATGG-3′ R: 5′-TGGGCCAGCCAGCACTAG-3′	Activation: 95 °C, 10 min	40 cycles	100 nM	NM_214055	[29]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
INSR	F: 5′-AAACGCCAGGGACATCGTCAAGG-3′ R: 5′-CCGCAGGGAACGCAGGTAACTCT-3′	Activation: 95 °C-10 min	40 cycles	200 nM	XM_005654749.1	[63]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
ITGAL	F: 5′-CTTGTCGAGCTGAAGGCTGA-3′ R: 5′-TTCCTGGTCCTTGGTGAGGA-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	NM_001044608.1	This study
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
LHB	F: 5′-TTCACCACCAGCATCTGTGC-3′ R: 5′-AAGAGGAGGCCTGGGAGTAG-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	XM_005664700.1	This study
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
MUC4	F: 5′-GATGCCCTGGCCACAGAA-3′ R: 5′-TGATTCAAGGTAGCATTCATTTGC-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	NM_001206344.2	[64]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
PRLR	F: 5′-CCAGATACCTAATGACTTCTCAATG-3′ R: 5′-TCCAACAGATGGGTGTCAAA-3′	Activation: 50 °C, 30 min;	40 cycles	500 nM	NM_214084	[63]
		95 °C, 15 min
		1. Denaturation: 94 °C, 15 s
		2. Annealing: 55 °C, 30 s
		3. Extension: 72 °C, 30 s
		77 °C, 15 s
PTGS1	F: 5′-CAACACTTCACCCACCAGTTCTTC-3′ R: 5′-TCCATAAATGTGGCCGAGGTCTAC-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	AF207823.1	[65]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 60 °C, 1 min
		3. Extension: 72 °C, 1 min
TGFA	F: 5′-CGCGCTGGGTATCTTGTTG-3′ R: 5′-GTGGGAATCTGGGCAGTCAT-3′	Activation: 50 °C, 2 min;	40 cycles	200 nM	NM_214251.1	[66]
		95 °C, 10 min
		1. Denaturation: 95 °C, 3 s
		2. Annealing: 60 °C, 30 s
		3. Extension: 72 °C, 1 min
ACTB	F: 5′-ACATCAAGGAGAAGCTCTGCTACG-3′ R: 5′-GAGGGGCGATGATCTTGATCTTCA-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	U07786	[67]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 61 °C, 1 min
		3. Extension: 72 °C, 1 min
GAPDH	F: 5′-CCTTCATTGACCTCCACTACATGG-3′ R: 5′-CCACAACATACGTAGCACCAGCATC-3′	Activation: 95 °C, 10 min	40 cycles	500 nM	U48832	[68]
		1. Denaturation: 95 °C, 15 s
		2. Annealing: 59 °C, 1 min