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. 2019 Mar 13;10(3):213. doi: 10.3390/genes10030213

Figure 2.

Figure 2

Reactive oxygen species (ROS) accumulation in the primary root tips with or without quercetin treatment. (A) The 3,3-diaminobenzidine (DAB) staining for H2O2 in the rice primary root tips with or without quercetin treatment. Experiments were repeated three times with similar results. Three to six roots were used for each experiment. Bars = 200 μm. (B) The DAB staining intensity in the rice primary root tips was determined with ImageJ. (C) NBT staining for O2− in rice primary root tips with or without quercetin treatment. Experiments were repeated three times with similar results. Three to six roots were used for each experiment. Bars = 200 μm. (D) The nitro blue tetrazolium (NBT) staining intensity in the rice primary root tips was determined with ImageJ. The asterisks in (B,D) indicate the differences between the untreated and quercetin-treated rice root tips (Student t test, ** p < 0.01).