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. 2019 Mar 1;10(3):190. doi: 10.3390/genes10030190

Figure 7.

Figure 7

Validation of the RNA-seq analysis using qRT-PCR. Among the CySNO-induced stem cell-related genes, six were selected based on the fold change (Fold change >4) in their expression levels and analyzed through qRT-PCR. The transcript accumulation of these genes was compared with the RNA-seq data, and the Pearson correlation coefficient was calculated using Microsoft Excel. R represents the Pearson correlation coefficient. All of the data points are the means of the three replicates, and the error bars represent ± SE.