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Effect of CLE-10 on autophagy-related proteins LC3-I/II, p-ULK1, ULk1, p62, mTOR, and p-mTOR were detected with and without autophagy inhibitor 3-MA, CQ, as well as the mTOR agonist rapamycin by Western blot analysis. (a) CLE-10 was used in combination with CQ. (b) CLE-10 was used in combination with 3-MA. (c) CLE-10 was used in combination with rapamycin. (* p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control group or the CLE-10 group).
