Figure 6.

The effect of tartary buckwheat extract on muscle mitochondrial gene expression and sirtulin 1 (SIRT1) enzyme activity. The mRNA levels of PGC-1α (A) and SIRT1 (B) were analyzed by real-time PCR and normalized to GAPDH. SIRT enzyme activity was measured by a colorimetric SIRT activity assay kit (C). Data are expressed as mean ± SEM (n = 8). Values with different letters are significantly different at p < 0.05. NOR: normal diet; HFD: 45% high-fat diet; TB-L: HFD + 0.5% TB; TB-H: HFD + 1.0% TB.