Table 3.

Variance analysis for ACE inhibitory activity in the RSM test.

Source	Sum of Squares	Df	Mean Square	F Value	p Value	Significance
Model	262.15	9	29.13	39.67	<0.0001	**
A-pH	72.35	1	72.35	98.53	<0.0001	**
B-Temperature	3.04	1	3.04	4.13	0.0815
C-Substrate concentration	0.51	1	0.51	0.7	0.4301
AB	40.66	1	40.66	55.38	0.0001	**
AC	25.73	1	25.73	35.04	0.0006	**
BC	0.021	1	0.021	0.029	0.8705
A2	88.03	1	88.03	119.89	<0.0001	**
B2	22.78	1	22.78	31.03	0.0008	**
C2	1.45	1	1.45	1.98	0.2026
Residual	5.14	7	0.73
Lack of Fit	1.96	3	0.65	0.82	0.5449
Pure Error	3.18	4	0.79
Corrected Total	267.29	16

Df: degrees of freedom, MS: mean square, F and p values, respectively ** p < 0.001, extremely significant.