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. 2019 Mar 14;11(3):624. doi: 10.3390/nu11030624

Figure 2.

Figure 2

Stem cell-like characteristics of MDA-MB-231/IR cells. (A) Morphologies of MDA-MB-231 cells and MDA-MB-231/IR cells. (B,C) Migration and (D,E) invasion were analyzed on same number of MDA-MB-231 cells and MDA-MB-231/IR cells for 24 h. (F,G) Western blot assay for detection of EMT markers expressed in MDA-MB-231 cells and MDA-MB-231/IR cells. GAPDH was used as a control; band intensities were quantified using ImageJ. (H,I) Mammosphere formation over seven days, (J,K) expression of cell surface markers, and (L,M) side population (SP) were detected by FACS analyses. (N,O) Western blot assay for stem cell markers on MDA-MB-231 cells and MDA-MB-231/IR cells. Asterisks (*) indicate significant differences at p < 0.05.