Skip to main content
. 2019 Mar 18;20(6):1363. doi: 10.3390/ijms20061363

Figure 1.

Figure 1

Phytohaemagglutinin (PHA)-stimulation of Jurkat cells induces transcription factor EB (TFEB) nuclear translocation and exocytosis. (A) Immunoblot analysis of TFEB in cytosolic and nuclear fractions from resting (Rest) and PHA-stimulated (PHA) Jurkat cells. Cytosolic TFEB level was normalized over β-actin, whereas nuclear TFEB level was normalized over H3. Values are the mean ± SEM of three independent experiments. ** p < 0.01 and *** p < 0.001 (PHA-stimulated vs. resting cells). (B) Horseradish peroxidase (HRP) enzyme activity in culture medium from resting and PHA-stimulated cells. Values are the mean ± SEM of three independent experiments. *** p < 0.001 (PHA-stimulated vs. resting cells).