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. 2019 Mar 18;20(6):1363. doi: 10.3390/ijms20061363

Figure 7.

Figure 7

Curcumin analogue C1 promotes the recruitment of Hex and Gal on lipid microdomains. Lipid microdomains were isolated from starved (Starv), curcumin (Cur, 5 μM). and curcumin analogue C1 (C1, 1 μM) 24 h-treated and untreated (CTRL) SH-SY5Y cells. (A) Collected fractions were analyzed by Dot blotting for the lipid microdomain maker GM1 by using the cholera toxin B subunit. Representative Dot blotting of five independent experiments is reported. (B) Collected fractions were also analyzed by immunoblotting for the lipid microdomain maker flotillin 2 (Flot-2). Representative immunoblotting of five independent experiments is reported. (C) Total Hex, Hex A, and Gal enzymatic activities in the GM1-enrhiched fractions 2–4 are reported as total mU (tot. mU). Values are the mean ± SEM of five independent experiments. *** p < 0.001 (treated or starved vs. untreated cells, CTRL). LM, lipid microdomain fractions; H, high-density fractions.