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. 2019 Apr 1;19(5):4035–4042. doi: 10.3892/mmr.2019.10112

Figure 1.

Figure 1.

Suppression of miR-214 attenuates the viability of MDA-MB-231 cells. (A and B) Expression levels of Hsa-miR-214 and α1-AT in normal tissues, TBNC tissues and BC tissues, as determined by RT-qPCR. **P<0.01 vs. Normal tissues (C) Binding sites of Hsa-miR-214 and α1-AT, as predicted by microRNA.org. (D) miR-214 mimics and α1-AT-3′UTR or α1-AT-3′UTR mut plasmids were co-transfected into 293 cells using Lipofectamine® 2000. Luciferase activity was measured by luciferase reporter assay. **P<0.01 vs. miR-NC + α1-AT-3′UTR group; ##P<0.01 vs. miR-214 mimics + α1-AT-3′UTR group. (E) mRNA expression levels of α1-AT in cells were assessed using RT-qPCR. **P<0.01 vs. control and NC cells. (F) Protein expression levels of α1-AT in cells were evaluated by western blotting. **P<0.01 vs. control and NC cells. (G) mRNA expression levels of miR-214 in MDA-MB-231 cells following transfection with miR-214 mimics were assessed using RT-qPCR. **P<0.01 vs. control and NC cells. (H) Expression levels of miR-214 in MDA-MB-231 cells following transfection with miR-214 inhibitors were assessed by RT-qPCR. **P<0.01 vs. control and NC cells. (I) Cell viability was determined using Cell Counting Kit-8. *P<0.05, **P<0.01 vs. 6 h; #P<0.05 vs. 12 h. 3′UTR, 3′-untranslated region; α1-AT, α1-AT, α1-antitrypsin; BC, breast cancer; miR-214, microRNA-214; mut, mutant; NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TNBC, triple-negative BC.