Table 3.
Extraction efficiency of spiked plasmid DNA from EDTA collection tubes and urine.
| Day 1 | ||||||||
| Spiked SD in 1 mL of Plasma Or Urine | Plasma | Urine | ||||||
| SET1 | SET2 | SET1 | SET2 | |||||
| Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | |
| Spiked_SD4.0 | 8575 ± 1429 | 97.4% | 8908 ± 1518 | 87.3% | 7100 ± 463 | 80.7% | 6375 ± 130 | 62.5% |
| Spiked_SD4.5 | 4579 ± 386 | 86.5% | 5026 ± 44 | 92.7% | 4173 ± 518 | 78.8% | 4559 ± 235 | 84.1% |
| Spiked_SD5.0 | 1088 ± 16 | 79.4% | 1118 ± 46 | 82.5% | 990 ± 13 | 72.2% | 982 ± 29 | 72.5% |
| Spiked_SD5.5 | 660 ± 32 | 93.6% | 646 ± 47 | 91.0% | 553 ± 22 | 78.3% | 558 ± 22 | 78.6% |
| Spiked_SD6.0 | 115 ± 7.8 | 80.0% | 105 ± 5.7 | 66.9% | 104 ± 1.3 | 71.9% | 111 ± 5.9 | 71.0% |
| Spiked_SD6.5 | 62 ± 2.1 | 76.2% | 57 ± 0.7 | 85.0% | 60 ± 3.7 | 74.0% | 59 ± 2.5 | 88.4% |
| Spiked_SD7.0 | 11 ± 2.6 | 85.3% | 12 ± 1.4 | 90.4% | 11 ± 2.3 | 81.5% | 9.6 ± 1.0 | 72.7% |
| Spiked_SD7.5 | 6.3 ± 1.1 | 99.4% | 5.6 ± 0.6 | 75.4% | 4.7 ± 0.4 | 73.3% | 5.4 ± 0.5 | 73.1% |
| Spiked_SD8.0 | 1.6 ± 0.5 | 108.6% | 1.4 ± 0.5 | 97.3% | 1.2 ± 0.6 | 81.6% | 0.7 ± 0.3 | 50.4% |
| Spiked_SD8.5 | 0.7 ± 0.6 | 93.6% | 0.7 ± 0.2 | 102.2% | 0.6 ± 0.5 | 91.2% | 0.5 ± 0.1 | 78.9% |
| Day 2 | ||||||||
| Spiked SD in 1 mL of Plasma Or Urine | Plasma | Urine | ||||||
| SET1 | SET2 | SET1 | SET2 | |||||
| Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | Recovered Conc. | Recovery Rate | |
| Spiked_SD4.0 | 9167 ± 989 | 99.2% | 8417 ± 792 | 74.2% | 6675 ± 218 | 72.3% | 7292 ± 823 | 64.3% |
| Spiked_SD4.5 | 5279 ± 539 | 97.2% | 5494 ± 251 | 93.2% | 3488 ± 540 | 64.2% | 3915 ± 72 | 66.4% |
| Spiked_SD5.0 | 1174 ± 59 | 90.9% | 1146 ± 37 | 83.9% | 1008 ± 44 | 78.0% | 911 ± 10 | 66.7% |
| Spiked_SD5.5 | 535 ± 24 | 78.7% | 532 ± 23 | 75.4% | 513 ± 35 | 75.5% | 480 ± 33 | 68.1% |
| Spiked_SD6.0 | 113 ± 2.9 | 73.1% | 108 ± 12 | 82.9% | 98 ± 3.0 | 63.2% | 97 ± 7.0 | 74.2% |
| Spiked_SD6.5 | 57 ± 2.8 | 79.8% | 56 ± 1.5 | 77.0% | 51 ± 4.1 | 70.9% | 49 ± 7.0 | 67.9% |
| Spiked_SD7.0 | 12 ± 1.7 | 118.1% | 12 ± 0.5 | 91.6% | 10 ± 1.4 | 98.9% | 9.6 ± 2.5 | 75.2% |
| Spiked_SD7.5 | 4.8 ± 0.6 | 72.5% | 4.6 ± 0.3 | 63.1% | 5.0 ± 0.5 | 75.0% | 4.6 ± 1.5 | 63.1% |
| Spiked_SD8.0 | 0.8 ± 0.7 | 102.7% | 1.4 ± 0.3 | 82.6% | 1.6 ± 0.9 | 211.9% | 0.8 ± 0.6 | 49.8% |
| Spiked_SD8.5 | 0.5 ± 0.3 | 118.4% | 0.5 ± 0.1 | 89.1% | 0.8 ± 0.3 | 195.4% | 0.2 ± 0.4 | 40.6% |
Recovered conc.: Copy number concentration (copy/μL) of DNA soutions extracted from 1.01 mL of spiked samples. Recovered rate (%) = (Recovered conc. × 90 μL of elution buffer)/(10 μL × Spiked_SD conc.) × 100.