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. 2019 Mar 16;11(3):641. doi: 10.3390/nu11030641

Figure 2.

Figure 2

Inhibition of cyanidin-induced TRPM6 and CNNM4 expression by GW6471. (A) MCE301 cells were incubated with vehicle, 10 μM GW9662, 10 μM GW6471, 10 μM GSK3787, or 10 μM H-89 in the presence and absence of 10 μM cyanidin for 6 h. Quantitative real-time PCR was performed using primer pairs for TRPM6, CNNM4, and β-actin. The contents of TRPM6 and CNNM4 mRNAs are represented relative to the values in vehicle. (B) MCE301 cells were incubated with vehicle, 10 μM GW9662 or 10 μM H-89 in the presence and absence of 10 μM cyanidin-3G for 6 h. Quantitative real-time PCR was performed using primer pairs for TRPM6, CNNM4, and β-actin. The contents of TRPM6 and CNNM4 mRNAs are represented relative to the values in vehicle. n = 4. ** p < 0.01 and NS p > 0.05 compared with vehicle. ## p < 0.01 and # p < 0.05 compared without cyanidin or cyanidin-3G alone.