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. 2019 Mar 16;11(3):641. doi: 10.3390/nu11030641

Figure 6.

Figure 6

Association of PPARα with PPRE in the TRPM6 and CNNM4 promoters. MCE301 cells were incubated with vehicle or 10 μM GW6471 in the absence and presence of 10 μM cyanidin for 2 h. Genomic DNA was immunoprecipitated with an anti-PPARα antibody. After immunoprecipitation, the 5′-flanking region of TNRP6 was amplified by semi-quantitative (A) and quantitative PCR (B) using primer pairs amplifying PPRE from TRPM6 and CNNM4. Input was amplified by the primer without immunoprecipitation. ChIP data are represented relative to the values in cyanidin alone. n = 3.