Skip to main content
. 2019 Mar 22;20(6):1467. doi: 10.3390/ijms20061467

Figure 1.

Figure 1

Zoledronate increased receptor activator of nuclear factor kB ligand (RANKL) and sclerostin mRNA expressions in MLO-Y4 cells. MLO-Y4 cells were cultured in the presence of indicated doses of clodronate (C, 0.1 or 1 µM) or zoledronate (Z, 0.1 or 1 µM) for 48 h. (a,b) Effects of increasing concentrations of zoledronate on MLO-Y4 cells. Cell Counting Kit-8 (CCK-8) assay showed that zoledronate did not reduce cell viability at all concentrations. (c–f) At the end of culture, total RNAs were isolated and the mRNA expressions of (c) Macrophage-colony stimulating factor (M-CSF), (d) RANKL, (e) angiogenin (ANG), and (f) sclerostin were examined by quantitative real-time PCR analyses. CON refers to the control vehicle, phosphate-buffered saline (PBS). The quantitative data are presented as mean ± SD (* p < 0.05).