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Expression of wild type LM-UOX and mutants in E. coli. Expression was done at 25 °C, 30 °C and 37 °C then whole cell lysate (W), which contains both soluble and insoluble protein fractions and soluble extract (S), which represents only soluble protein, were prepared and run on SDS-PAGE. a–f represent wild type, S136C, D288C, N289C, I27C/N289C and A132C/A225C, respectively. Red arrows indicate the overexpressed target proteins.
