Table 3.
Specific activity, kinetic parameters and production yield of WT and LM-UOX mutants.
| LM-UOX and Variants |
Specific Activity (unit/mg) |
Km (µM) | kcat (s−1) |
kcat/Km (µM−1 s−1) |
Yield (mg/1-L Culture) |
|---|---|---|---|---|---|
| WT | 10.45 ± 0.14 | 18.43 ± 0.4 | 29.89 ± 0.3 | 1.62 | 132.1 |
| S136C | 7.88 ± 0.08 | 15.28 ± 0.1 | 22.49 ± 0.1 | 1.47 | 130.0 |
| D288C | 9.38 ± 0.09 | 189.65 ± 0.2 | 65.07 ± 0.3 | 0.34 | 5.8 |
| N289C | 10.38 ± 0.10 | 30.46 ± 0.1 | 34.06 ± 0.1 | 1.12 | 13.2 |
| I27C/N289C | 9.75 ± 0.05 | 35.39 ± 0.1 | 32.58 ± 0.2 | 0.92 | 100.5 |
| A132C/A225C | 15.38 ± 0.12 | 39.19 ± 0.5 | 58.19 ± 0.6 | 1.49 | 40.5 |
Values are means ± standard deviation of the replicates.