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. 2019 Mar 13;20(6):1269. doi: 10.3390/ijms20061269

Table 4.

Primers for LM-UOX cDNA amplification and site-directed mutagenesis. NdeI and XhoI restriction sites were included in forward and reverse primers (underlined text), respectively.

Primers Description DNA sequence (5’→3′)
LM-UOX FP a for construction
of pET20LM-UOX		 GGTCTTCCATATGTCCCACTATGTTTCC
LM-UOX RP b CCGCTCGAGCAGTTTGGAATGTGCC
I27C FP for mutagenesis
of I27→C27		 GGCTACGGGAAAAACTGCGTAAAAGTCTTGCAC
I27C RP GTGCAAGACTTTTACGCAGTTTTTCCCGTAGCC
A132C FP for mutagenesis
of A132→C132		 GCATGTGCATTGCTTTATTTACAGTCC
A132C RP GGACTGTAAATAAAGCAATGCACATGC
S136C FP for mutagenesis
of S136→C136		 GCATGCTTTTATTTACTGTCCAGAAGCAACTCGG
S136C RP CGAGTTGCTTCTGGACAGTAAATAAAAGCATG
A225C FP for mutagenesis
of A225→C225		 ATTGAGAAGTTTTGTGGCCCTTACGAGTG
A225C RP CACTCGTAAGGGCCACAAAACTTCTCAAT
D288C FP for mutagenesis
of D288→C288		 GTTCTGCTGCCTCTGTGCAACCCATCTGGCAAC
D288C RP GTTGCCAGATGGGTTGCACAGAGGCAGCAGAAC
N289C FP for mutagenesis
of N289→C289		 CTGCTGCCTCTGGACTGCCCATCTGGCAACATTAC
N289C RP GTAATGTTGCCAGATGGGCAGTCCAGAGGCAGCAG

a FP = Forward primer, b RP = Reverse primer.