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. 2019 Mar 14;19(5):3527–3536. doi: 10.3892/mmr.2019.10034

Figure 5.

Figure 5.

miR-1 suppresses the expression of HSP60 by binding to its 3′UTR. (A) Sequence alignment between miR-1 and the 3′UTR of human, rat and mouse HSP60. The matched base pairs are connected by a vertical line. (B) Effects of carvedilol on HSP60 mRNA levels in myocardial tissues, as measured by RT-qPCR. (C) Effects of carvedilol on HSP60 protein expression levels in myocardial tissues, as measured by western blot analysis. (D) Effects of carvedilol on HSP60 mRNA levels in cardiomyocytes, as measured by RT-qPCR. (E) Effects of carvedilol on HSP60 protein expression levels in cardiomyocytes, as measured by western blot analysis. Expression levels were normalized to that of GAPDH. All data are presented as relative levels. *P<0.05 vs. Sham or Con; #P<0.05 vs. MI or H2O2; +P<0.05 vs. +Car. Car, carvedilol; Con, control; MI, myocardial infarction; HSPD1/HSP60, heat shock protein 60; miR, microRNA; 3′UTR, 3′untranslated region; RT-qPCR, reverse transcription quantitative polymerase chain reaction.