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. 2019 Mar 22;20(6):1458. doi: 10.3390/ijms20061458

Figure 5.

Figure 5

Changes in GFB-related proteins in mice after DSS administration. Immunofluorescent microscopy (A) and Western blot analysis of protein expression (B) against synaptopodin (A-a, A-a’; B-a; 100 kDa), podocalyxin (A-b, A-b’; B-b; 130 kDa), nephrin (A-c, A-c’; B-c; 185 kDa) and VE-cadherin (A-d, A-d’; B-d; 130 kDa) in glomeruli were conducted for control and DSS-colitis mice. (B) Representative bands (left), and relative band intensity ratios (right) were analyzed. (C) Illustration of GFB-related proteins changes in this study. All values are means ± SEM (n = 6), * p < 0.05 and ** p < 0.01 vs. control. Scale bars = 10 μm (A-a, A-a’, A-b, A-c, A-c’); 20 μm (A-b’, A-d, A-d’). Abbreviation: SYNPO, synaptopodin; PODXL, podocalyxin; VE-Cad, VE-cadherin; FP, foot processes.