Table 1.
Composition of duodenal and bile juices in the simulated small intestinal fluid.
| Duodenal Juice | Bile Juice | |||
|---|---|---|---|---|
| Inorganic components | 40 mL NaCl | 175.3 g/L | 30 mL NaCl | 175.3 g/L |
| 40 mL NaHCO3 | 84.7 g/L | 68.3 mL NaHCO3 | 84.7 g/L | |
| 10 mL KH2PO4 | 8 g/L | 4.2 mL KCl | 89.6 g/L | |
| 6.3 mL KCl | 89.6 g/L | |||
| 10 mL MgCl2 | 5 g/L | |||
| 250 μL HCl 35% | 350 μL HCl 35% | |||
| Organic components | 4 mL urea | 25 g/L | 10 mL urea | 25 g/L |
| Mixture of organic and inorganic solution 1 | 9 mL CaCl2 | 16.8 g/L | 10 mL CaCl2 | 16.8 g/L |
| 1 g BSA | 1.8 g BSA | |||
| 15 g bile salts 2 | ||||
| pH | 6.4 ± 0.2 | 6.4 ± 0.2 | ||
1 Mixture of inorganic and organic solution was set up to 500 mL with distilled water. 2 The 15 g of bile salts were replaced by 30 g of bile extract in the simulated model of small intestinal digestion.