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. 2019 Jan 1;8(1):2. doi: 10.3390/pathogens8010002

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ocular pathology of parental and double mutant S. aureus strain Newman and their culture supernatants in rabbit corneas and the protein contributing to virulence. (A) The SLE score at 24 h PI of S. aureus strain Newman double mutant (ΔhlaΔhlg; ) lacking alpha- and gamma-toxins, which produced substantial pathology as compared to normal eyes, was significantly less than that of the wild-type strain ( ) (P ≤ 0.001). When their culture supernatants were injected directly into the corneal stroma, the SLE score of the double mutant at 24 h after injection was substantial compared to normal eyes, but significantly lower than that of the wild-type strain (P = 0.004). (B) Non-denaturing (no SDS) zymogram of the pooled fractions of concentrated culture supernatant of the S. aureus double mutant (Newman ΔhlaΔhlg) containing toxic activities to the rabbit cornea. (C) A zymogram with SDS showing standard proteins (Lane 1) and the extracted toxin (Lane 2).

Inline graphic — Ocular pathology of parental and double mutant S. aureus strain Newman and their culture supernatants in rabbit corneas and the protein contributing to virulence. (A) The SLE score at 24 h PI of S. aureus strain Newman double mutant (ΔhlaΔhlg; ) lacking alpha- and gamma-toxins, which produced substantial pathology as compared to normal eyes, was significantly less than that of the wild-type strain ( ) (P ≤ 0.001). When their culture supernatants were injected directly into the corneal stroma, the SLE score of the double mutant at 24 h after injection was substantial compared to normal eyes, but significantly lower than that of the wild-type strain (P = 0.004). (B) Non-denaturing (no SDS) zymogram of the pooled fractions of concentrated culture supernatant of the S. aureus double mutant (Newman ΔhlaΔhlg) containing toxic activities to the rabbit cornea. (C) A zymogram with SDS showing standard proteins (Lane 1) and the extracted toxin (Lane 2).