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. 2019 Jan 1;8(1):2. doi: 10.3390/pathogens8010002

Figure 3.

Figure 3

Recombinant SSL1 proteolytic activity and polymerization. (A) SDS-PAGE showing the standards (left lane), the dimer form of the recombinant protein at ~46 kDa, and the monomer at ~23 kDa (right lane). (B) Photographs of rabbit corneas injected with heat-inactivated (left photo) or active recombinant SSL1 (12 µg) at 24 h after injection (right photo). (C) Analysis of purified SSL1 including zymography (standards in Lane 1; recombinant SSL1 in Lane 2) and a Western blot of recombinant SSL1 in Lane 3. Please note that the protease activity is at the molecular size of a dimer (46 kDa), but not at the monomer size (23 kDa) and that both the monomer and dimer reacted with the antibody.