Figure 1.
(a) Forward scatter plotted versus anti-stage specific embryonic antigen SSEA1–fluorescein isothiocyanate (FITC) fluorescence for cESC showing an identifiable SSEA1–positive cESC sub-population. Left: Unstained control. (b) Phase contrast microscopy image of chicken embryonic stem cells (cESC) culture (magnification 20×; Evos Fl microscope). (c) Fold change in expression by qRT-PCR of cESC pluripotency markers during viral infection and stimulation with recombinant chIFN-α. Values are normalised against DF-1 mock-infected cells. Error bars represent SEM (n = 3).