Table 1.
Primer sequence used for real-time quantitative PCR.
| Gene | Forward (5′–3′) | Reverse (5′–3′) | |
|---|---|---|---|
| HepG2 | PPARγ | TGCAGGTGATCAAGAAGACG | AGTGCAACTGGAAGAAGGGA |
| C/EBPα | TGGACAAGAACAGCAACGAGTA | ATTGTCACTGGTCAGCTCCAG | |
| SREBP-1c | GCGCCTTGACAGGTGAAGTC | GCCAGGGAAGTCACTGTCTTG | |
| FAS | CCCCTGATGAAGAAGGATCA | ACTCCACAGGTGGGAACAAG | |
| CPT-1 | CCTCCGTAGCTGACTCGGTA | GGAGTGACCGTGAACTGAAA | |
| PPARα | ACGATTCGACTCAAGCTGGT | GTTGTGTGACATCCCGACAG | |
| β-actin | CTCTTCCAGCCTTCCTTCCT | AGCACTGTGTTGGCGTACAG | |
| Mouse Liver | PPARγ | CAGGAGAGCAGGGATTTGCA | CCTACGCTCAGCCCTCTTCAT |
| C/EBPα | TTACAACAGGCCAGGTTTCC | GGCTGGCGACATACAGTACA | |
| SREBP-1c | ATCGCAAACAAGCTGACCTG | AGATCCAGGTTTGAGGTGGG | |
| FAS | TTGCTGGCACTACAGAATGC | AACAGCCTCAGAGCGACAAT | |
| CPT-1 | CTCAGTGGGAGCGACTCTTCA | GGCCTCTGTGGTACACGACAA | |
| PPARα | CAGGAGAGCAGGGATTTGCA | CCTACGCTCAGCCCTCTTCAT | |
| β-actin | CTGTCCCTGTATGCCTCTG | ATGTCACGCACGATTTCC |
PPARγ, peroxisome proliferator-activated receptor gamma; C/EBPα, CCAAT/enhancer-binding protein alpha; SREBP-1c, sterol regulatory element-binding protein1-c; FAS, fatty acid synthase; CPT-1, carnitine palmitoyltransferase-1; PPARα, Peroxisome proliferator-activated receptor alpha.