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Effect of hypoxia on (A) LCN2 gene expression and (B) protein levels of LCN2 in cultured primary astrocytes isolated from the cerebral cortex. (A) RT-qPCR analysis reveals a significant increase in LCN2 mRNA levels at 2 h post hypoxia. (B) LCN2 protein concentrations measured in supernatant of cultured hypoxic astrocytes at 2 h post hypoxia also shows a significant upregulation of LCN2. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001. Norm, normoxia; Hypo, Hypoxia.
