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. 2019 Mar 13;20(6):1271. doi: 10.3390/ijms20061271

Figure 4.

Figure 4

Effect of reoxygenation after an initial 2 h hypoxia on the mRNA expression profiles of LCN2 and various pro- and anti-inflammatory cytokines in mouse astrocyte cultures from the cerebral cortex of WT and LCN2-deficient animals. (A) LCN2 expression is significantly but temporarily induced in response in WT astrocytes 3 and 6 h after reoxygenation and returned to control level at 12 h reoxygenation. (B–D) The profiles of HIF-1α, TNF-α, and IL-6 mRNA expression mainly follow the LCN2 pattern in WT astrocytes. Such an induction of HIF-1α, TNF-α and IL-6 expression was not observed in LCN2-deficient astrocytes. (E) In contrast, the anti-inflammatory cytokine IL-10 was significantly elevated in LCN2-deficient astroglia but only moderately elevated in WT glia. Rep, reoxygenation (reperfusion). * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001. The number of animals included for reoxygenation was n = 5 per time point. Norm, normoxia; Hypo, Hypoxia.