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Weight of rats (A) and the concentration of copper ion (B) and in the spleen of each group of rats treated with oral administration of copper-containing preparations for 28 consecutive days. * p < 0.05 and ** p < 0.01 vs. control. Values represent means ± SD (N = 10). A: Control group, 1% w/v HPMC; B: micro-Cu group, 200 mg/kg BW; C: CuCl2 group, 200 mg/kg CuCl2 solution; D: nano-Cu low group, 50 mg/kg; E: nano-Cu medium group, 100 mg/kg; F: nano-Cu high group, 200 mg/kg.
