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. 2019 Mar 12;11(3):116. doi: 10.3390/pharmaceutics11030116

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) ATP produced by MC3T3-E1 cells grown in the presence of the following hydrogels: CS (black), CS-HACS (light grey) and CS-HACS-AgNWs (dark grey). Cultures grown on tissue-culture plates were taken as control (white). Data are reported as mean ± SD (n = 3). One-way ANOVA returned p < 0.0001 for 7 and 14 days; (B) ALP expression by MC3T3-E1 cells grown in the presence of the same hydrogels. Data reported as mean ± SD (n = 3). One-way ANOVA returned p < 0.0001 for 7 days and p = 0.0005 for 14 days. The results of the post-hoc Tukey multi comparison test for (A,B) are reported in the graph: ****, p < 0.0001; ***, p < 0.001; **, p < 0.01 compared to the control; ^#, p < 0.05, ^## p < 0.001, ^### p < 0.001.