Table 2.
Effect of Gcn4p on conversion at HIS4
| Percent
conversion at HIS4 ± SE (events/total tetrads)
|
||||
|---|---|---|---|---|
| Leu+ Lys− | Leu− Lys− | Leu+ Lys+ | Leu− Lys+ | |
| Ade+ | ||||
| GCN4/GCN4 | 27% ± 1.7*‡ (195/719) | 21% ± 1.7*§ (126/588) | 19% ± 1.5†‡ (127/682) | 13% ± 1.5†§ (72/534) |
| Δgcn4/Δgcn4 | 12% ± 1.9¶ (37/300) | 10% ± 1.8¶ (28/274) | 13% ± 1.9¶ (40/314) | 10% ± 1.7 (33/313) |
| GCN4–URA3–GCN4/GCN4 | ND | ND | ND | 13 ± 2.4 (25/191) |
| GCN4c–URA3–GCN4/GCN4 | ND | ND | ND | 48 ± 2.1‖ (94/197) |
| Ade−** | ||||
| GCN4/GCN4 | 40% ± 3.5 (78/196) | 37% ± 3.6 (64/175) | ND | 37% ± 3.6 (69/185) |
| Δgcn4/Δgcn4¶ | 29% ± 3.4 (52/180) | 23% ± 3.1 (42/181) | ND | 23% ± 3.6 (32/140) |
ND, not determined.
*
Prototrophy for leucine elevates conversion frequency significantly in lysine auxotrophs.
†
Prototrophy for leucine elevates conversion frequency significantly in lysine prototrophs.
‡
Auxotrophy for lysine elevates conversion frequency significantly in leucine prototrophs.
§
Auxotrophy for lysine elevates conversion frequency significantly in leucine auxotrophs.
¶
Conversion frequency is significantly lower in Δgcn4 strains than in GCN4.
‖
Constitutive expression of Gcn4p elevates conversion frequencies significantly.
**
Auxotrophy for adenine elevates conversion frequency significantly (independently of phenotype for leucine or lysine).