Table 1.
Protein purification results of Saz_CA. WAU: Wilbur–Anderson unit.
| No. | Purification Step | Enzyme Activity [WAU mL−1] | Total Activity [WAU] | Protein Concentration [mg L−1] | Specific Activity [WAU mgprot−1] | Purification Fold | Yield |
|---|---|---|---|---|---|---|---|
| 1 | Saz_CA Crude lysate | 312.33 | 46,849 | 598.68 | 521.69 | 1.00 | 100% |
| 2 | Saz_CA Heat treatment 60 °C 30 min | 478.28 | 71,741 | 544.39 | 878.55 | 1.68 | 153% |
| 3 | Saz_CA Heat treatment 70 °C 30 min | 505.91 | 75,887 | 311.46 | 1624.36 | 3.11 | 162% |
| 4 | Saz_CA Ni2+ affinity chromatography | 300.79 | 30,079 | 35.19 | 8546.74 | 16.38 | 64% |