Table 2.
Protein purification results of Saz_CA-ChBD.
| No. | Purification step | Enzyme Activity [WAU mL−1] | Total Activity [WAU] | Protein Concentration [mg L−1] | Specific Activity [WAU mgprot−1] | Purification Fold | Yield |
|---|---|---|---|---|---|---|---|
| 1 | Saz_CA-ChBD Crude lysate | 132.73 | 19,909 | 586.11 | 226.45 | 1.00 | 100% |
| 2 | Saz_CA-ChBD Heat treatment 60 °C 30 min | 263.61 | 39,542 | 553.75 | 476.04 | 2.10 | 199% |
| 3 | Saz_CA-ChBD Heat treatment 70 °C 30 min | 196.75 | 29,513 | 345.42 | 569.60 | 2.52 | 148% |
| 4 | Saz_CA-ChBD Ni2+ affinity chromatography | 152.26 | 15,227 | 45.36 | 3357.09 | 14.82 | 76% |