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. 2019 Mar 5;19(5):3575–3583. doi: 10.3892/mmr.2019.10004

Figure 2.

Figure 2.

miR-145 inhibits ovarian cancer cell proliferation, cell cycle, invasion and migration. (A) SKOV3 and ES-2 cells were transfected with miR-145 mimics or the control mimics, and the expression of miR-145 was determined by reverse transcription quantitative polymerase chain reaction. (B) Cell viability in cells transfected with miR-145 mimics or NC was measured by Cell Counting Kit-8 assay at 4 time points (0, 24, 48, 72 and 96 h). UV-visible absorbance was measured at 450 nm. *P<0.05 and **P<0.01 for mimic control and miR-145 mimic groups vs. mock group. (C) Wound healing assay was performed at 24 h post-transfection (magnification, 200×). (C-a) SKOV3 cells at 0 h. Following 24 h, the migration ability of SKOV3 cells transfected with (C-b) miR-NC significantly increased compared with (C-c) cells transfected with miR-145. The results indicated that overexpression of miR-145 inhibits cell migration. (D) Invasion assays of ovarian cancer cells transfected with miR-145 or the control mimics. (E) The expression levels of Bcl-2 and Bax were detected in SKOV3 and ES-2 cells using western blot analysis. Experiments were performed in triplicate. *P<0.05 and **P<0.01. miR, microRNA; NC, negative control; FC, fold change.