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. 2019 Mar 18;20(6):1360. doi: 10.3390/ijms20061360

Table 4.

Kinetic constants of purified dextranase from Penicillium cyclopium.

Dextran T5 b Dextran T70 b Dextran T2000 b
Km (mM) 2.72 ± 0.04 2.61 ± 0.02 2.45 ± 0.01
Vmax (µmol Glucose min−1 mg−1 Protein) 189.50 ± 0.03 192.70 ± 0.04 203.21 ± 0.01
Kacat (s−1) 208.45 ± 0.01 211.97 ± 0.02 223.53 ± 0.03
Kcat/Km (M−1 s−1) 76636.0 ± 0.06 81214.6 ± 0.03 91236.7 ± 0.05

aKcat value was calculated assuming a molecular mass of 66 kDa for native dextranase. b The results reported were the means of three replications ± SD.