Figure 6.

Effect of plant-derived smoke on redox-homeostasis related proteins in maize. Maize seeds were treated without or with 1000, 2000, and 4000 ppm plant-derived smoke for 6 h and grown for 4 days. Proteins extracted from shoot were separated by SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidine difluoride membranes. The membranes were incubated with anti-RuBisCO large/small subunits and anti-RuBisCO activase antibodies. The relative intensities of bands were calculated using ImageJ software. Data are shown as means ± S.D. from 3 independent biological replicates. Different letters indicate that the change is statistically significant as determined by one-way ANOVA followed by Tukey’s multiple comparison (p ˂ 0.05). In images, “M” is used for marker.