Figure 7.

Effect of plant-derived smoke on enzymatic analysis and expression level of ascorbate peroxidase and peroxiredoxin gene in maize shoot. Maize seeds were treated without or with 1000 ppm, 2000 ppm, and 4000 ppm plant-derived smoke for 6 h and sown. Activities of ascorbate peroxidase (APX) and peroxiredoxin (PRX) were measured in 4-day old maize shoot (A). For gene expression level, RNAs extracted from maize shoot at 3rd day of treatment were analyzed by qRT-PCR (B). Relative mRNA abundances of ascorbate peroxidase and peroxiredoxin were normalized against 18S rRNA abundance. The data are shown as means ± S.D. from 4 independent biological replicates. White and black bars indicate ascorbate peroxidase (APX) and peroxiredoxin (PRX), respectively. Different letters indicate that the change is significant as determined by one-way ANOVA followed by Tukey’s multiple comparison (p ˂ 0.05).