Table 3.
Frequency of gene conversion after vegetative passaging and sexual reproduction
| Strain* | F1
individual
|
F1
subculture
|
F2 progeny
|
|||
|---|---|---|---|---|---|---|
| Lines† | Frequency‡ | Lines | Frequency | Lines | Frequency | |
| NC174 | 1 × 22 | 0.076 a | 4 × 25 | 0.043 b | 4 × 20 | 0.071 a |
| NC123 | 1 × 33 | 0.15 a | 4 × 25 | 0.12 a | 2 × 20 | 0.28 b |
| NC15B | 1 × 20 | 0.15 a | 4 × 25 | 0.32 b | 6 × 20 | 0.40 c |
F1 individual refers to the strains analyzed in Table 1. The frequencies in F1 subcultures are averages of four independent cultures of the F1 individual passaged five times. F2 progeny are 2–6 progeny obtained by selfing the F1 individual.
m × n indicates that n zoospore lines were analyzed from each of m individuals, subcultures, or F2 progeny.
The frequency of conversions per locus per zoospore line averaged among 2–6 individuals including loci with no changes and excluding loci homozygous in the parent F1 zoospore line (NC174.6, NC123.10, or NC15B.4). Twelve loci were analyzed in NC174 and its derivatives and 10 each for NC123 and NC15B. Comparing across the table, frequencies with the same postscript (a, b, and c) were not significantly different (P > 0.10) by the χ2 test. Frequencies with different postscripts were significantly different (P < 0.01).