Table 4.
Gene conversion during vegetative passage
F1 hybrid subculture† | Lines analyzed | Lines homozygous for each marker*
|
||||||
---|---|---|---|---|---|---|---|---|
21J17L | 8801 | 8127 | 4141 | B16 | 19N3R | 4N8R | ||
NC123 | 33 | 0 | 15 (6) | 13 (6) | 6 (6) | 4 (6) | ||
NC123.10.A | 25 | 21 (6) | 0 | 0 | 0 | 0 | ||
NC123.10.B | 25 | 19 (6) | 0 | 3 (6) | 0 | 0 | ||
NC123.10.C | 25 | 21 (6) | 0 | 11 (6) | 0 | 0 | ||
NC123.10.D | 25 | 17 (6) | 18 (6) | 1 (6) | 0 | 12 (7) | ||
NC15B | 20 | 8 (6) | 0 | 5 (6) | 17 (7) | 0 | ||
NC15B.4.A | 25 | 4 (6) | 0 | 23 (6) | 0 | 20 (6) | ||
NC15B.4.B | 25 | 17 (6) | 20 (6) | 22 (6) | 9 (7) | 21 (6) | ||
NC15B.4.C | 25 | 22 (6) | 12 (6) | 21 (6) | 23 (7) | 21 (6) | ||
NC15B.4.D | 25 | 19 (6) | 3 (6) | 22 (6) | 19 (7) | 22 (6) |
Markers are as described in Table 1. Markers K14, 6802, M4, AP4, and AP19 showed no changes.
One single zoospore line from each F1 progeny was selected (e.g. NC123.10), and four independent subcultures (A–D) were established from it (see Materials and Methods).