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. 2019 Mar 14;19(5):3604–3614. doi: 10.3892/mmr.2019.10022

Figure 5.

Figure 5.

miR-512-5p target to regulate the expression of ETS1. (A) Reverse transcription quantitative polymerase chain reaction and (B) western blot analysis were used to detect ETS1 following transfection with mimics miR-512-5p in A549 and H1299 cells. Luciferase activity was analyzed following co-transfection of (C) A549 and (D) H1299 cells with pGL3-ETS1-3′-UTR, pGL3-ETS1-3′-UTR-mut and miR-512-5p or NC mimics. Data are presented as mean ± standard deviation. **P<0.01 vs. NC. ETS1, E26 transformation specific-1; miR, microRNA; NC, negative control; UTR, untranslated region; hsa, Homo sapiens; mut, mutant.