. 2019 Jan 27;8(1):14. doi: 10.3390/pathogens8010014

Table 3.

Primers used for the generation of knockout mutants.

Primer	Nucleotide Sequence (5’ – 3’)	Fragment Length (bp)	Target Gene
FliF_F	CGGTACCCGGGGATCGAACAGATCAACTACCAGCG	771	Flagellar M-ring gene, fliF
FliF_R	CGACTCTAGAGGATCTTGATGTGGCTGATGGTG		Flagellar M-ring gene, fliF
AxyE_F	CGGTACCCGGGGATCGACGTCAAGGAAAACCAG	795	RND-type multidrug resistance efflux pump gene, axyE
AxyE_R	CGACTCTAGAGGATCGGGCAACTGTTCGATCTT		RND-type multidrug resistance efflux pump gene, axyE
MetC_F	CGGTACCCGGGGATCTGATGCAGGACAAGGAGT	809	Cystathionine beta-lyase gene, metC
MetC_R	CGACTCTAGAGGATCTAGGCGTAGGTGTCGTAG		Cystathionine beta-lyase gene, metC
M14F^a	CCAGGGTTTTCCCAGTCACGA
M14R^a	GCGGATAACAATTTCACACAGGA

Underlined sequences comprise 15 bases of homology with the ends of the linearized vector pUC19. ^a M14F and M14R primers are located in the vector adjacent to the insertion site.