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. 2019 Mar 19;10(3):231. doi: 10.3390/genes10030231

Figure 1.

Figure 1

Porcine Satellite Cells (PSCs) culture, differentiation, and immunofluorescence detection. (A) The proliferation PSCs (PRO) were differentiated for 4 days in differentiation medium (D1, D2, and D4), and myotubes aroused at D2, with their size increasing over time. Scale bars: 200 μm. Magnification: 100×. (B) Immunofluorescence detection of four marker genes in proliferation cells (PRO, upper panel), and D2 differentiated cells (DIF, bottom panel). Antibodies were indicated as green (left to right: PAX7, MYOD1) and red (left to right: MYOSIN, MYOG), Hoechst33342 dye was blue. Scale bars: 200 μm. Magnification: 100×.